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Transnuclear CD 8 T cells specific for the immunodominant epitope Gra6 lower acute‐phase Toxoplasma gondii burden
Author(s) -
Sanecka Anna,
Yoshida Nagisa,
Dougan Stephanie K.,
Jackson John,
Shastri Nilabh,
Ploegh Hidde,
Blanchard Nicolas,
Frickel EvaMaria
Publication year - 2016
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/imm.12643
Subject(s) - toxoplasma gondii , epitope , biology , t cell receptor , virology , antigen , t cell , monoclonal antibody , immune system , microbiology and biotechnology , antibody , immunology
Summary We generated a CD 8 T‐cell receptor ( TCR ) transnuclear ( TN ) mouse specific to the L d ‐restricted immunodominant epitope of GRA 6 from Toxoplasma gondii as a source of cells to facilitate further investigation into the CD 8 T‐cell‐mediated response against this pathogen. The TN T cells bound L d ‐Gra6 tetramer and proliferated upon unspecific and peptide‐specific stimulation. The TCR beta sequence of the Gra6‐specific TN CD 8 T cells is identical in its V‐ and J‐region to the TCR ‐ β harboured by a hybridoma line generated in response to Gra6 peptide. Adoptively transferred Gra6 TN CD 8 T cells proliferated upon Toxoplasma infection in vivo and exhibited an activated phenotype similar to host CD 8 T cells specific to Gra6. The brain of Toxoplasma ‐infected mice carried Gra6 TN cells already at day 8 post‐infection. Both Gra6 TN mice as well as adoptively transferred Gra6 TN cells were able to significantly reduce the parasite burden in the acute phase of Toxoplasma infection. Overall, the Gra6 TN mouse represents a functional tool to study the protective and immunodominant specific CD 8 T‐cell response to Toxoplasma in both the acute and the chronic phases of infection.