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Transient receptor potential vanilloid 1 expression and function in splenic dendritic cells: a potential role in immune homeostasis
Author(s) -
Assas Mushref Bakri,
Wakid Majed H.,
Zakai Haytham A.,
Miyan Jaleel A.,
Pennock Joanne L.
Publication year - 2016
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/imm.12562
Subject(s) - transient receptor potential channel , homeostasis , immune system , microbiology and biotechnology , function (biology) , receptor , transient (computer programming) , biology , immunology , neuroscience , genetics , computer science , operating system
Summary Neuro‐immune interactions, particularly those driven by neuropeptides, are increasingly implicated in immune responses. For instance, triggering calcium‐channel transient receptor potential vanilloid 1 ( TRPV 1) on sensory nerves induces the release of calcitonin‐gene‐related peptide ( CGRP ), a neuropeptide known to moderate dendritic cell activation and T helper cell type 1 polarization. Despite observations that CGRP is not confined to the nervous system, few studies have addressed the possibility that immune cells can respond to well‐documented ‘neural’ ligands independently of peripheral nerves. Here we have identified functionally relevant TRPV 1 on primary antigen‐presenting cells of the spleen and have demonstrated both calcium influx and CGRP release in three separate strains of mice using natural agonists. Furthermore, we have shown down‐regulation of activation markers CD 80/86 on dendritic cells, and up‐regulation of interleukin‐6 and interleukin‐10 in response to CGRP treatment. We suggest that dendritic cell responses to neural ligands can amplify neuropeptide release, but more importantly that variability in CGRP release across individuals may have important implications for immune cell homeostasis.