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Interferon‐ γ enhances both the anti‐bacterial and the pro‐inflammatory response of human mast cells to Staphylococcus aureus
Author(s) -
Swindle Emily J.,
Brown Jared M.,
Rådinger Madeleine,
DeLeo Frank R.,
Metcalfe Dean D.
Publication year - 2015
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/imm.12524
Subject(s) - staphylococcus aureus , microbiology and biotechnology , degranulation , biology , immune system , innate immune system , interferon , tumor necrosis factor alpha , cytokine , interferon gamma , immunology , interleukin 8 , receptor , bacteria , biochemistry , genetics
Summary Human mast cells (hu MC s) are involved in both innate and adaptive immune responses where they release mediators including amines, reactive oxygen species ( ROS ), eicosanoids and cytokines. We have reported that interferon‐ γ ( IFN ‐ γ ) enhances Fc γ R‐dependent ROS production. The aim of this study was to extend these observations by investigating the effect of IFN ‐ γ on the biological responses of hu MC s to Staphylococcus aureus . We found that exposure of hu MC s to S. aureus generated intracellular and extracellular ROS , which were enhanced in the presence of IFN ‐ γ . IFN ‐ γ also promoted bacteria killing, β ‐hexosaminidase release and eicosanoid production. Interferon‐ γ similarly increased expression of mRNA s encoding CCL 1 to CCL 4, granulocyte–macrophage colony‐stimulating factor ( GM ‐ CSF ), tumour necrosis factor‐ α and CXCL 8 in S. aureus ‐stimulated hu MC s. The ability of IFN ‐ γ to increase CXCL 8 and GM ‐ CSF protein levels was confirmed by ELISA . Fibronectin or a β 1 integrin blocking antibody completely abrogated IFN ‐ γ ‐dependent S. aureus binding and reduced S. aureus ‐dependent CXCL 8 secretion. These data demonstrate that IFN ‐ γ primes hu MC s for enhanced anti‐bacterial and pro‐inflammatory responses to S. aureus , partially mediated by β 1 integrin.