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The presence of interleukin‐27 during monocyte‐derived dendritic cell differentiation promotes improved antigen processing and stimulation of T cells
Author(s) -
Jung JooYong,
Roberts Lawton L.,
Robinson Cory M.
Publication year - 2015
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/imm.12417
Subject(s) - microbiology and biotechnology , immune system , cytokine , biology , antigen , dendritic cell , t cell , immunology , monocyte , cellular differentiation , acquired immune system , antigen presenting cell , interleukin 12 , cytotoxic t cell , in vitro , biochemistry , gene
Summary Dendritic cells ( DC s) are potent antigen‐presenting cells necessary to establish effective adaptive immune responses. The cytokine environment that exists at the time of DC differentiation may be an important but often ignored determinant in the phenotypic and functional properties of DC s. Interleukin‐27 ( IL ‐27) is a unique cytokine that has both inflammatory and immune suppressive activities. Although it can both promote and oppose activity of different T‐cell subsets, mostly anti‐inflammatory activity has been described toward macrophages and DC s. However, the specific effect of IL ‐27 during DC differentiation and how that may change the nature of the antigen‐presenting cell has not been investigated. In this report, we show that IL ‐27 treatment during monocyte‐derived DC differentiation enhanced the ability to process antigens and stimulate T‐cell activity. DC s differentiated in the presence of IL ‐27 showed enhanced acidification of latex bead‐containing phagosomes that was consistent with elevated expression of vacuolar‐ ATP ases. This resulted in inhibition of intracellular growth of Staphylococcus aureus . In addition, the levels of MHC class II surface expression were higher in DC s differentiated in the presence of IL ‐27. Production of IL ‐12 was also significantly increased during S. aureus infection of IL ‐27‐differentiated DC s. The net effect of these activities was enhanced CD 4 + T‐cell proliferation and T helper type 1 cytokine production. These findings are important to a wide number of immunological contexts and should be considered in the development of future vaccines.