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The CD 27 + memory B cells display changes in the gene expression pattern in elderly individuals
Author(s) -
Báez Alicia,
ÁlvarezLaderas Isabel,
Piruat José I.,
CaballeroVelázquez Teresa,
Barbado María Victoria,
MillánUclés África,
Medrano Mayte,
GarcíaGuerrero Estefanía,
SánchezAbarca Luis Ignacio,
PérezSimón José Antonio
Publication year - 2015
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/imm.12381
Subject(s) - gene expression , gene , biology , candidate gene , gene expression profiling , population , microbiology and biotechnology , genetics , medicine , environmental health
Summary Memory B cells ( MBC s) have a long lifespan compared with naive B cells ( NBC s), remaining viable for years. This could predispose them to suffer misbalances in the gene expression pattern in the long term, which might be involved in the development of age‐related B ‐cell disorders. In order to identify genes whose expression might change during life, we analysed the gene expression patterns of CD 27 − NBC s versus CD 27 + MBC s in young and old subjects. Using microarray assays we observed that the expression pattern of CD 27 − NBC s versus CD 27 + MBC s is significantly different. Furthermore, to evaluate the age effect, we compared the gene expression pattern of young versus aged subjects in both cell populations. Interestingly, we did not find significant differences in the CD 27 − NBC population between young and aged individuals, whereas we found 925 genes differentially expressed in CD 27 + MBC s. Among these genes, 193 were also differentially expressed in CD 27 + MBC s compared with CD 27 − NBC s, most of them involved in cell survival, cell growth and proliferation, cellular development and gene expression. We conclude that gene expression profiles of CD 27 − NBC s and CD 27 + MBC s are different. Moreover, whereas the gene expression pattern of CD 27 + MBC s varies with age, the same does not happen in CD 27 − NBC s. This suggests that MBC s undergo time‐dependent changes, which could underlie a higher susceptibility to dysfunction with age.