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Distinct B‐cell populations contribute to vaccine antigen‐specific antibody production in a transgenic mouse model
Author(s) -
O Eunju,
Ko EunJu,
Kim MinChul,
Lee YoungTae,
Song JaeMin,
Kwon YoungMan,
Compans Richard W.,
Kang SangMoo
Publication year - 2014
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/imm.12287
Subject(s) - biology , virology , antibody , antigen , immunization , germinal center , b cell , vaccination , isotype , immunology , monoclonal antibody
Summary The generation of memory B cells by vaccination plays a critical role in maintaining antigen‐specific antibodies and producing antibody responses upon re‐exposure to a pathogen. B‐cell populations contributing to antibody production and protection by vaccination remain poorly defined. We used influenza virus‐like particle ( VLP ) vaccine in a transgenic mouse model that would identify germinal centre‐derived memory B cells with the expression of yellow fluorescent protein ( YFP + cells). Immunization with influenza VLP vaccine did not induce significant increases in YFP + cells although vaccine antigen‐specific antibodies in sera were found to confer protection against a lethal dose of influenza A virus (A/ PR 8). In addition, CD 43 + B220 − populations with low YFP + cells mainly contributed to the production of vaccine antigen‐specific IgG isotype‐switched antibodies whereas CD 43 − B220 + populations with high YFP + cells were able to produce vaccine antigen‐specific IgM antibodies. Challenge infection of immunized transgenic mice with live influenza A virus resulted in significant increases in YFP + cells in the B220 − populations of spleen and bone marrow cells. These results suggest that CD 43 + B220 − B cells generated by vaccination are important for producing influenza vaccine antigen‐specific antibodies and conferring protection.