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Lack of transglutaminase 2 diminished T‐cell responses in mice
Author(s) -
Kim JinHee,
Hong Jun Man,
Jeong Eui Man,
Lee Wang Jae,
Kim HangRae,
Kang Jae Seung,
Kim InGyu,
Hwang Youngil
Publication year - 2014
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/imm.12282
Subject(s) - t cell , microbiology and biotechnology , tissue transglutaminase , immune system , biology , ex vivo , antigen presenting cell , chemistry , in vivo , immunology , biochemistry , enzyme
Summary Transglutaminase 2 ( TG 2) has been reported to play a role in dendritic cell activation and B‐cell differentiation after immunization. Its presence and role in T cells, however, has not been explored. In the present study, we determined the expression of TG 2 on mouse T cells, and evaluated its role by comparing the behaviours of wild‐type and TG 2 −/− T cells after activation. In our results, naive T cells minimally expressed TG 2, expression of which was increased after activation. T‐cell proliferation, expression of activation markers such as CD 69 and CD 25, and secretions of interleukin‐2 and interferon‐ γ were suppressed in the absence of TG 2, presumably due, in part, to diminished nuclear factor‐ κ B activation. These effects on T cells seemed to be reflected in the in vivo immune response, the contact hypersensitivity reaction elicited by 2,4‐dinitro‐1‐fluorobenzene, with lowered peak responses in the TG 2 −/− mice. When splenic T cells from mice immunized with tumour lysate‐loaded wild‐type dendritic cells were re‐challenged ex vivo with the same antigen, the profile of surface markers including CD 44, CD 62L, and CD 127 strongly indicated lesser generation of memory CD 8 + T cells in TG 2 −/− mice. In the TG 2 −/− CD 8 + T cells, moreover, Eomes expression was markedly decreased. These results indicate possible roles of TG 2 in CD 8 + T‐cell activation and CD 8 + memory T‐cell generation.