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Melanoma differentiation‐associated gene 5 in zebrafish provoking higher interferon‐promoter activity through signalling enhancing of its shorter splicing variant
Author(s) -
Zou Peng Fei,
Chang Ming Xian,
Xue,
Liu Xue Qin,
Li Jun Hua,
Fu Jian Ping,
Chen Shan Nan,
Nie Pin
Publication year - 2014
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/imm.12179
Subject(s) - biology , interferon , zebrafish , transfection , gene , pathogen , enhancer , virus , microbiology and biotechnology , virology , gene expression , genetics
Summary Melanoma differentiation‐associated gene 5 ( MDA 5) is one of the three members in the retinoic acid‐inducible gene I‐like receptor ( RLR ) family, which are cytoplasmic pathogen recognition receptors recognizing intracellular viruses. In the present study, MDA 5 and its spliced shorter forms, named as MDA 5a and MDA 5b, were identified in zebrafish. MDA 5a and MDA 5b can be up‐regulated in cell lines following the infection of a negative ss RNA virus, the spring viraemia of carp virus ( SVCV ), and an intracellular Gram‐negative bacterial pathogen E dwardsiella tarda , implying that the RLR may also be able to sense elements released from bacteria. The over‐expression of MDA 5a and MDA 5b in fish cells resulted in significant induction of type I interferon promoter activity and enabled the protection of transfected cells against SVCV infection. Furthermore, the shorter spliced form, MDA 5b when co‐transfected with MDA 5a or mitochondrial antiviral signalling protein ( MAVS ), induced a significantly higher level of interferon promoter activity, indicating that MDA 5b may function as an enhancer in the interaction between MDA 5 and MAVS .