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CD 8 + CD 122 + regulatory T cells contain clonally expanded cells with identical CDR 3 sequences of the T ‐cell receptor β‐chain
Author(s) -
Okuno Yusuke,
Murakoshi Ayako,
Negita Masashi,
Akane Kazuyuki,
Kojima Seiji,
Suzuki Haruhiko
Publication year - 2013
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/imm.12067
Subject(s) - biology , cd8 , cytotoxic t cell , natural killer t cell , t cell receptor , interleukin 21 , il 2 receptor , antigen presenting cell , microbiology and biotechnology , zap70 , immune system , t cell , immunology , genetics , in vitro
Summary We identified CD 8 + CD 122 + regulatory T cells ( CD 8 + CD 122 + T reg cells) and reported their importance in maintaining immune homeostasis. The absence of CD 8 + CD 122 + T reg cells has been shown to lead to severe systemic autoimmunity in several mouse models, including inflammatory bowel diseases and experimental autoimmune encephalomyelitis. The T ‐cell receptors ( TCR s) expressed on CD 8 + CD 122 + T reg cells recognize the target cells to be regulated. To aid in the identification of the target antigen(s) recognized by TCR s of CD 8 + CD 122 + T reg cells, we compared the TCR diversity of CD 8 + CD 122 + T cells with that of conventional, naive T cells in mice. We analysed the use of TCR ‐ V β in the interleukin 10‐producing population of CD 8 + CD 122 + T cells marked by high levels of CD 49d expression, and found the significantly increased use of V β13 in these cells. Immunoscope analysis of the complementarity‐determining region 3 ( CDR 3) of the TCR β‐chain revealed remarkable skewing in a pair of V β regions, suggesting the existence of clonally expanded cells in CD 8 + CD 122 + T cells. Clonal expansion in V β13 + cells was confirmed by determining the DNA sequences of the CDR 3s. The characteristic TCR found in this study is an important building block for further studies to identify the target antigen recognized by CD 8 + CD 122 + T reg cells.