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Ragweed pollen extract intensifies lipopolysaccharide‐induced priming of NLRP 3 inflammasome in human macrophages
Author(s) -
Varga Aliz,
Budai Marietta M.,
Milesz Sándor,
Bácsi Attila,
Tőzsér József,
Benkő Szilvia
Publication year - 2013
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/imm.12052
Subject(s) - inflammasome , lipopolysaccharide , nadph oxidase , reactive oxygen species , microbiology and biotechnology , caspase 1 , proinflammatory cytokine , inflammation , chemistry , biology , immunology
Summary Ragweed pollen extract ( RWE ) possesses intrinsic NADPH oxidase activity that induces oxidative stress by initiating the production of intracellular reactive oxygen species ( ROS ). The ROS are important contributors to the manifestation of allergic inflammation; furthermore, concomitant exposure to an allergen and an endotoxin trigger a stronger inflammatory response. One of the main pro‐inflammatory cytokines produced in inflammatory responses is interleukin‐1β ( IL ‐1β), and its production is associated with caspase‐1‐containing inflammasome complexes. Intracellular ROS have been implicated in NLRP 3 inflammasome‐mediated IL ‐1β production, therefore, we aimed to study whether RWE influences the function of NLRP 3 inflammasome. Here we describe that, in the presence of NADPH , RWE significantly elevates lipopolysaccharide‐induced IL ‐1β production of THP ‐1 cells as well as human primary macrophages and dendritic cells. We also demonstrate that increased IL ‐1β production is mediated through NLRP 3 inflammasome in THP ‐1 macrophages. We provide evidence that RWE elevates cytosolic ROS level in these cells, and ROS inhibitors abolish IL ‐1β production. Furthermore, we show that RWE enhances lipopolysaccharide‐induced gene transcription/expression of pro‐ IL ‐1β and key components of the inflammasome via a ROS ‐dependent mechanism.