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Human cord blood γδ T cells expressing public Vγ2 chains dominate the response to bisphosphonate plus interleukin‐15
Author(s) -
Cairo Cristiana,
Sagnia Bertrand,
Cappelli Giulia,
Colizzi Vittorio,
Leke Rose G. F.,
Leke Robert J.,
Pauza C. David
Publication year - 2013
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/imm.12039
Subject(s) - cord blood , peripheral blood mononuclear cell , cytotoxic t cell , t cell , population , immune system , stimulation , immunology , myeloid , biology , chemistry , microbiology and biotechnology , endocrinology , medicine , in vitro , biochemistry , environmental health
Summary Compared with adults, the circulating Vγ2Vδ2 T ‐cell population in cord blood is present at low levels and does not show the strong bias for Vγ2‐Jγ1.2 rearrangements. These features may be a result of limited exposure to stimulatory phosphoantigens, lack of T ‐cell‐derived interleukin‐2 ( IL ‐2) or both. In cord blood mononuclear cell cultures, a single round of stimulation, using aminobisphosphonates to elevate phosphoantigen levels, resulted in expansion of adult‐like Vγ2 chains and accumulation of memory cells with cytotoxic potential. Selection was similar using IL ‐2 or myeloid‐derived IL ‐15. The Vγ2Vδ2 T cells present in neonates are capable of generating potent immune responses even when relying on IL ‐15.