Premium
THE PARTIAL ABSORPTION TEST : A NEW TECHNIQUE FOR TITRATING RH ANTIBODIES OF PATHOLOGICAL SIGNIFICANCE
Author(s) -
VOS G. H.
Publication year - 1958
Publication title -
australasian annals of medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.596
H-Index - 70
eISSN - 1445-5994
pISSN - 0571-9283
DOI - 10.1111/imj.1958.7.4.340
Subject(s) - titer , antibody , coombs test , dilution , medicine , chemistry , blocking antibody , rh blood group system , absorption (acoustics) , immunology , materials science , physics , composite material , thermodynamics
Summary A new test is described which enables the quantity of Rh antibody free in the fœtal serum to be estimated by titrations carried out on the maternal serum. Equal volumes of a hæmolysed 25% suspension of Rh‐positive red cells and a 1 : 4 dilution of the maternal anti‐Rh sera are incubated at room temperature for 15 minutes. The Rh antibody remaining free at the end of this time is titrated by the indirect antiglobulin method. The titre value obtained is referred to as the partial absorption (P.A.) titre. The P.A. titre is closely related to the titre of free Rh antibody in the cord serum of the infant at birth. The P.A. titre also is highly correlated with the blocking titre, but shows no relationship with the conglutination or albumin titres. The P.A. test, however, is more sensitive than the blocking test in detecting Rh antibodies of pathological significance, and is likely to be therefore of greater prognostic value.