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FLUORESCEIN‐GLOBULIN AFFINITIES FOR RED CELLS IN HIEMOLYTIC ANÆMIA
Author(s) -
HUGHES P. E.
Publication year - 1958
Publication title -
australasian annals of medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.596
H-Index - 70
eISSN - 1445-5994
pISSN - 0571-9283
DOI - 10.1111/imj.1958.7.3.228
Subject(s) - staining , gamma globulin , globulin , fluorescein , antibody , immunology , biology , pathology , chemistry , medicine , fluorescence , physics , quantum mechanics
Summary Red corpuscles, which normally are not affected, were found to become stained by fluorescein‐globulin in a small number of cases of lymphatic leukæmia. These cases were complicated by a hæmolytic anaemia associated with a positive Coombs reaction. Cases of congenital hæmolytic anæmia failed to show positive staining. Among other forms of acquired hæmolytic anæmia, positive staining was sometimes encountered in the presence of a positive Coombs reaction. This was demonstrated to be of a non‐specific type except in one case, complicating lupus erythematosus, in which it appeared to have an immunological mechanism. A faint staining and fluorescence was found in association with erythroblastosis fœtalis. Here the prior application of unconjugated anti‐human‐gamma‐globulin globulin to blood films resulted in enhancement rather than inhibition of staining. The non‐specific type of staining appears to be a physico‐chemical protein‐protein interaction which is independent of any immunological reaction.