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Changes in blood monocyte Toll‐like receptor and serum surfactant protein A reveal a pathophysiological mechanism for community‐acquired pneumonia in patients with type 2 diabetes
Author(s) -
Que Y.,
Shen X.
Publication year - 2016
Publication title -
internal medicine journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.596
H-Index - 70
eISSN - 1445-5994
pISSN - 1444-0903
DOI - 10.1111/imj.12978
Subject(s) - medicine , pathophysiology , tlr2 , immunology , pneumonia , peripheral blood mononuclear cell , type 2 diabetes mellitus , diabetes mellitus , receptor , tlr4 , endocrinology , biology , biochemistry , in vitro
Background The lung is one of the target organs of microangiopathy in diabetes mellitus ( DM ); patients with type 2 diabetes mellitus ( T2DM ) are vulnerable to pneumonia, and a variety of pathophysiological mechanisms has been described. Aim This study aimed to determine the pathophysiological mechanism of community‐acquired pneumonia ( CAP ) in T2DM patients. Methods A total of 90 individuals was included in this study comprised of three groups ( n = 30): healthy control, T2DM and T2DM + CAP groups. Toll‐like receptor ( TLR )2 and 4 protein and messenger RNA expression in peripheral blood monocytes( PBMC ) was assessed by western blot and reverse transcription–polymerase chain reaction, respectively, and surfactant protein A ( SP ‐A) levels were examined in serum samples by enzyme‐linked immunosorbent assay. Results In T2DM and T2DM + CAP groups, levels of both TLR2 /4 protein and mRNA in PBMC were decreased compared with controls ( P <0.05), with lower levels observed in the T2DM + CAP group in comparison with T2DM patients ( P <0.05). The serum SP ‐A levels in T2DM + CAP individuals were significantly higher than the values obtained for T2DM patients ( P <0.05). It also showed apparent increases when compared with that in controls although no statistical significance was detected. Conclusion In T2DM patients with pneumonia, TLR2 /4 levels in PBMC and serum SP ‐A were altered, maybe playing an important role in the susceptibility to pneumonia in T2DM patients.