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Identification and characterization of the long noncoding RNA Dreg1 as a novel regulator of Gata3
Author(s) -
Chan Wing Fuk,
Coughlan Hannah D,
Iannarella Nadia,
Smyth Gordon K,
Johanson Timothy M,
Keenan Christine R,
Allan Rhys S
Publication year - 2021
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1111/imcb.12408
Subject(s) - gata3 , enhancer , regulator , biology , gene , computational biology , genome , transcription factor , non coding rna , regulation of gene expression , genetics , promoter , rna , gene expression , microbiology and biotechnology
This study explores T‐cell‐specific genome organization and reveals a long noncoding RNA ( Dreg1 ) in an enhancer of the Gata3 gene. We characterize the full sequence of Dreg1 and find that its expression is highly correlated with Gata3 expression throughout early T‐cell development and in T helper type 2 differentiation. Inhibition and overexpression of Dreg1 in T‐ and B‐cell lines suggest that it may be involved in the establishment, but not in the maintenance of Gata3 expression.