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Necrotic cell death increases the release of macrophage migration inhibitory factor by monocytes/macrophages
Author(s) -
Dankers Wendy,
Hasnat Md Abul,
Swann Vanesa,
Alharbi Arwaf,
Lee Jacinta PW,
Cristofaro Megan A,
Gantier Michael P,
Jones Sarah A,
Morand Eric F,
Flynn Jacqueline K,
Harris James
Publication year - 2020
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1111/imcb.12376
Subject(s) - pyroptosis , macrophage migration inhibitory factor , necroptosis , inflammasome , macrophage , lipopolysaccharide , cytokine , programmed cell death , tumor necrosis factor alpha , immunology , cytotoxic t cell , microbiology and biotechnology , caspase 1 , inflammation , chemistry , biology , apoptosis , in vitro , biochemistry
Macrophage migration inhibitory factor (MIF) is a pleiotropic inflammatory molecule with both cytokine and noncytokine activity. MIF is constitutively released from multiple cell types via an unconventional secretory pathway that is not well defined. Here, we looked at MIF release from human and mouse monocytes/macrophages in response to different stimuli. While MIF release was not significantly altered in response to lipopolysaccharide or heat‐killed Escherichia coli , cytotoxic stimuli strongly promoted release of MIF. MIF release was highly upregulated in cells undergoing necrosis, necroptosis and NLRP3 inflammasome‐dependent pyroptosis. Our data suggest that cell death represents a major route for MIF release from myeloid cells. The functional significance of these findings and their potential importance in the context of autoimmune and inflammatory diseases warrant further investigation.