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Host CD8α + and CD103 + dendritic cells prime transplant antigen‐specific CD8 + T cells via cross‐dressing
Author(s) -
Li Bin,
Lu Chunni,
Oveissi Sara,
Song Jing,
Xiao Kun,
Zanker Damien,
Duan Mubin,
Chen Jianxin,
Xu Huji,
Zou Quanming,
Wu Chao,
Yewdell Jonathan W,
Chen Weisan
Publication year - 2020
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1111/imcb.12342
Subject(s) - priming (agriculture) , cytotoxic t cell , cd8 , antigen , immunology , t cell , transplantation , biology , microbiology and biotechnology , in vitro , immune system , medicine , biochemistry , botany , germination
Abstract The participation of dendritic cells (DCs) in CD8 + T‐cell‐mediated allograft rejection is a long‐standing question of great clinical relevance for tissue transplantation. Here, we show that Batf3 −/− mice demonstrate delayed allo‐skin graft rejection and are deficient in priming allo‐specific CD8 + T cells. Batf3 −/− mouse priming is restored by transferring either purified CD8α + or CD103 + DCs, demonstrating the critical role of these cells in alloreactivity. Using D b ‐restricted antiviral F5 transgenic T‐cell receptor T cells, which we demonstrate are alloreactive with H‐2K d , we show that cross‐dressing of CD8α + and CD103 + primes CD8 + T‐cell or allo‐specific responses in vitro and in vivo . These findings suggest novel strategies for moderating tissue rejection based on interfering with DC cross‐dressing or subsequent interaction with T cells.