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Regulatory T cells differ from conventional CD 4 + T cells in their recirculatory behavior and lymph node transit times
Author(s) -
Tong Alexander A,
Forestell Benjamin,
Murphy Daniel V,
Nair Aditya,
Allen Frederick,
Myers Jay,
Klauschen Frederick,
Shen Connie,
Gopal Angelica A,
Huang Alex Y,
Mandl Judith N
Publication year - 2019
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1111/imcb.12276
Subject(s) - transit time , node (physics) , lymph node , transit (satellite) , chemistry , physics , biology , immunology , engineering , public transport , transport engineering , quantum mechanics
Regulatory T cells (Tregs) continuously suppress autoreactive immune responses within tissues to prevent autoimmunity, yet the recirculatory behavior of Tregs between and within tissues enabling the maintenance of peripheral tolerance remains incompletely defined. Here, we quantified homing efficiency to and the dwell time of Tregs within secondary lymphoid organs ( SLO s) and used intravital two‐photon microscopy to measure Treg surveillance behavior of dendritic cells. Tregs homed substantially less efficiently to SLO s compared with conventional CD 4 + T cells (Tconvs), despite similar expression of homing receptors. Tregs remained on average 2–3 times longer within the LN than Tconvs before exiting, and retained Tregs differed from recirculating Tregs in phenotype, motility and interaction duration with dendritic cells. Taken together, these data revealed fundamental differences in Treg versus conventional T cell in vivo recirculation and migration behaviors, identified a Treg population with prolonged LN dwell time, and provided quantitative insight into their spatiotemporal behavior within LN s.