Premium
Chemosensory proteins participate in insecticide susceptibility in Rhopalosiphum padi , a serious pest on wheat crops
Author(s) -
Peng X.,
Qu M. J.,
Wang S. J.,
Huang Y. X.,
Chen C.,
Chen M. H.
Publication year - 2021
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/imb.12683
Subject(s) - rhopalosiphum padi , imidacloprid , biology , pest analysis , cypermethrin , aphid , gene , pesticide , toxicology , acetamiprid , agronomy , botany , aphididae , genetics , homoptera
Rhopalosiphum padi is a worldwide agricultural pest. Chemosensory proteins (CSPs) are considered to be a type of transporters which can bind chemicals from external environments. Previous research showed that the expression of some insect CSPs were significantly increased after exposure to insecticides, and CSPs were involved in insecticide resistance or susceptibility. However, the role of CSPs in the susceptibility and response of R. padi to insecticides is still unknown. In this study, we identified eight CSP ( RpCSP ) from R. padi by genome‐wide investigation. Seven RpCSP genes had two exons, while RpCSP7 had three exons. qPCR analyses showed that the mRNA levels of the eight RpCSP genes were significantly affected by imidacloprid and beta‐cypermethrin in different post‐treatment periods. Molecular docking predicted that there were hydrogen bonding sites which played key roles in binding of RpCSP4 , RpCSP5 , RpCSP6 , RpCSP7 and RpCSP10 with imidacloprid and beta‐cypermethrin. Knockdown of RpCSP4 , RpCSP5 , RpCSP6 and RpCSP10 by RNA interference significantly increased the aphid mortality under two sublethal concentrations of imidacloprid. Mortalities under two sublethal concentrations of beta‐cypermethrin conditions were significantly higher after injection of R. padi with ds CSP4 and ds CSP6 . The results indicate that some RpCSP genes are involved in the insecticide susceptibility of R. padi .