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Facilitating fructose‐driven metabolism exerts a protective effect on anoxic stress in Drosophila
Author(s) -
Kim H.,
Kim M.,
Kim M. S.
Publication year - 2021
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/imb.12667
Subject(s) - fructose , anoxic waters , biology , microbiology and biotechnology , gene , glycolysis , metabolism , drosophila melanogaster , carbohydrate metabolism , hypoxia (environmental) , biochemistry , chemistry , ecology , organic chemistry , oxygen
Hypoxic stress is linked to various cardiovascular disorders (e.g., stroke, myocardial infarction), mediated, at least in part, by a reduction in ATP synthesis. Fructose‐driven glycolysis is proposed as an alternative pathway capable of sustaining ATP production even under anoxic conditions. Here, we tested the hypothesis that facilitating fructose‐driven metabolism exerts a protective effect against anoxic stress in Drosophila . Genetically modified flies with the human fructose transporter (GluT5) and ketohexokinase (KHK) genes downstream of upstream activating sequence (UAS) were constructed. The GAL4‐UAS system was confirmed to: (i) increase the expression of GluT5 and KHK in a tissue‐specific and a time‐dependent manner (i.e., whole flies [with Act5c‐gene switch GAL4 driver], neurons [with elav‐gene switch GAL4 driver]) and (ii) reduce mortality of flies when placed under anoxic stress. Taken together, these data suggest that increasing fructose metabolism may be a clinically relevant approach to minimize hypoxia‐induced cellular damage.