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Molecular characterization, expression pattern and RNAi ‐mediated silencing of vitellogenin receptor gene in almond moth, Cadra cautella
Author(s) -
Husain M.,
Rasool K. G.,
Tufail M.,
Aldawood A. S.
Publication year - 2020
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/imb.12646
Subject(s) - vitellogenin , rna interference , biology , gene silencing , rna silencing , complementary dna , gene , rapid amplification of cdna ends , reverse transcription polymerase chain reaction , gene expression , real time polymerase chain reaction , microbiology and biotechnology , messenger rna , rna , genetics , molecular cloning
The almond moth is an important pest of date fruits worldwide. The females produce several eggs; however, role of vitellogenin receptor (VgR) in oocyte development by mediating endocytosis of major yolk protein precursor Vg remains yet unexplored. To investigate the role of vitellogenin receptor ( VgR ) in reproduction, Cadra cautella vitellogenin receptor ( CcVgR ) transcript was obtained using rapid amplification of cDNA ends‐polymerase chain reaction. Expression analysis of CcVgR was performed using reverse transcriptase and quantitative polymerase chain reaction (qPCR) in different developmental stages. RNA interference was performed by injecting CcVgR‐ based double‐stranded (ds)RNA at different exposure times. The results revealed that CcVgR is 5421 bp long, encoded 1807 amino acid, belongs to low‐density lipoprotein receptor superfamily and contains all conserved domains. Expression analysis confirmed that CcVgR is sex‐specific and starts to express in female larvae on day 19. Additionally, RNA interference (RNAi) of CcVgR‐ based dsRNA inhibited CcVgR expression up to 83% after 72 h, reduced fecundity and hatchability, and confirmed involvement of CcVgR in C. cautella reproduction. This report provides a basis for gene silencing in this species, and proposes RNAi technology potential for pest management.