Krüppel homologue 1 interacts directly with Hairy and regulates ecdysis in the brown planthopper | Zendy

Mao Y. | Zendy; Li Y. | Zendy; Gao H. | Zendy; Lin X. | Zendy

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Krüppel homologue 1 interacts directly with Hairy and regulates ecdysis in the brown planthopper

Author(s) -

Mao Y.,

Li Y.,

Gao H.,

Lin X.

Publication year - 2020

Publication title -

insect molecular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.955

H-Index - 93

eISSN - 1365-2583

pISSN - 0962-1075

DOI - 10.1111/imb.12635

Subject(s) - krüppel , gene knockdown , biology , rna interference , downregulation and upregulation , juvenile hormone , transcription factor , transcription (linguistics) , microbiology and biotechnology , zinc finger , homomeric , ecdysone receptor , gene , genetics , biochemistry , rna , nuclear receptor , protein subunit , hormone , linguistics , philosophy

Juvenile hormone (JH) plays important roles in the growth and development of insects. JH and its receptor methoprene‐tolerant (Met) regulate the expression of transcription factors to control the transcription of downstream genes. The expression of Hairy ( Hry ) and Krüppel homologue 1 ( Kr‐h1 ) is regulated by JH and JH receptors. Hry and Kr‐h1 are both crucial in mediating JH signalling. However, whether they interact at the gene level in regulating metamorphosis and whether they interact physically at the protein level remain unknown. We used co‐immunoprecipitation, glutathione S‐transferase pull‐down and RNA interference (RNAi) approaches to study the genetic and biochemical interactions of the two proteins Hry and Kr‐h1. The results showed that brown planthopper ( Nilaparvata lugens ) Hry and Kr‐h1 interact directly: Hry binds to the N‐terminal of Kr‐h1, which includes five zinc‐finger domains. The RNAi experiment showed that downregulation of Hry reduced the ratio of ecdysis failure caused by knockdown of Kr‐h1 , indicating that the downregulation of Hry might mitigate ecdysis failure via the downregulation of Kr‐h1 . The expression of Hry increased significantly when Kr‐h1 was downregulated, whereas it did not change significantly when both were downregulated. Our results suggest that the binding of Hry protein with Kr‐h1 prevents the N‐terminal five zinc‐finger domains from binding with DNA, which in turn inactivates the transcription activator or inhibitor function of Kr‐h1. Hry could possibly be used as a target for pesticide applications in the future.

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