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Glutathione transferase modulates acute ethanol‐induced sedation in Drosophila neurones
Author(s) -
Choi H.J.,
Cha S. J.,
Kim K.
Publication year - 2019
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/imb.12547
Subject(s) - glutathione , glutathione s transferase , ethanol , gene knockdown , biology , dopaminergic , pharmacology , serotonergic , rna interference , sedation , biochemistry , gene , microbiology and biotechnology , dopamine , toxicology , neuroscience , enzyme , serotonin , rna , receptor
Heavy alcohol consumption leads to neuropathological damage and alcohol use disorder, which affects the health of people and results in a cost burden. However, the genes modulating sensitivity to ethanol remain largely unknown. Here, we identified a novel gene, Drosophila glutathione transferase omega 1 ( GstO1 ), which plays a critical role in regulating sensitivity to ethanol sedation. GstO1 mutant flies showed highly increased ethanol sensitivity. Furthermore, the expression level of GstO1 regulates the behavioural response to ethanol, because decreasing and increasing GstO1 affects sedation sensitivity in a contrasting manner. In addition, the RNA interference‐mediated knockdown of GstO1 expression reveals that GstO1 mediates sensitivity to ethanol sedation in neurones, including dopaminergic and serotonergic neurones. Altogether, our findings provide the first evidence for the involvement of glutathione transferase in the response to alcohol in Drosophila and provide a novel mechanistic insight into the toxicity and sensitivity of ethanol exposure.