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Dusky works upstream of Four‐jointed and Forked in wing morphogenesis in Tribolium castaneum
Author(s) -
Li C.,
Li B.,
Ma S.,
Lü P.,
Chen K.
Publication year - 2017
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/imb.12327
Subject(s) - rna interference , morphogenesis , wing , biology , drosophila melanogaster , gene knockdown , microbiology and biotechnology , gene silencing , phenocopy , gene , rna , phenotype , genetics , engineering , aerospace engineering
Dusky ( dy ) is required for cytoskeletal reorganization during wing morphogenesis in Drosophila melanogaster , but which genes participate together with dy for wing morphogenesis has remained unclear. In Tribolium castaneum , dy is highly expressed at the late embryonic stage. Tissue‐specific expression analysis indicated high expression levels of dy in the epidermis, head and fat body of late‐stage larvae. RNA interference (RNAi) targeting dy significantly decreased adult wing size and caused improper folding of the elytra. Meanwhile, dy knockdown reduced the transcription of four‐jointed ( fj ) and forked ( f ). Our results show that fj RNAi reduces adult wing size and that silencing f results in abnormal wing folding in T. castaneum . Interestingly, knocking down fj and f simultaneously phenocopies dy RNAi, suggesting that dy probably acts upstream of fj and f to regulate wing morphogenesis in T. castaneum .