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First protein and peptide characterization of the tarsal adhesive secretions in the desert locust, Schistocerca gregaria , and the M adagascar hissing cockroach, Gromphadorhina portentosa
Author(s) -
Betz O.,
Maurer A.,
Verheyden A. N.,
Schmitt C.,
Kowalik T.,
Braun J.,
Grunwald I.,
Hartwig A.,
Neuenfeldt M.
Publication year - 2016
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/imb.12241
Subject(s) - schistocerca , desert locust , locust , biology , proteome , western blot , secretion , cockroach , biochemistry , botany , ecology , gene
Peptides and proteins have been largely neglected in the analysis of insect tarsal adhesives. After extraction of the protein fraction of the tarsal secretion of the desert locust, Schistocerca gregaria , and Madagascar hissing cockroach, Gromphadorhina portentosa , we combined Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS‐PAGE) and matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐TOF MS) analyses for protein mass detection. In both these insects, SDS‐PAGE analysis revealed several protein bands ranging from 8–190 kDa in both the tarsal secretion and the tibia control sample. Two ( S. gregaria ) and one ( G. portentosa ) protein bands exclusively occurred in the tarsal secretion and can be considered to belong to peptides and proteins specific to this secretion. MALDI‐TOF analyses revealed 83 different proteins/peptides of 1–7 kDa in S. gregaria , and 48 of 1–11 kDa in G. portentosa . 59 ( S. gregaria ) and 27 ( G. portentosa ) proteins exclusively occurred in the tarsal secretion. In G. portentosa , a characteristic series of signal peaks occurred in the range of c . 10–12 kDa, each peak being approximately 160 Da apart. Such a pattern is indicative of proteins modified by glycosylation. Our approach demonstrates that extensive sampling involving considerable time and manpower to sample the adhesive fluid directly from the tarsi opens up a perspective for extracting peptides and proteins in sufficient quantities. This makes them accessible to the field of proteomics and thus to elucidate their possible function in the adhesive process.