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Shifts in B uchnera aphidicola density in soybean aphids ( A phis glycines ) feeding on virus‐infected soybean
Author(s) -
Cassone Bryan J.,
Redinbaugh Margaret G.,
Dorrance Anne E.,
Michel Andrew P.
Publication year - 2015
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/imb.12170
Subject(s) - buchnera , biology , soybean aphid , fecundity , aphid , obligate , host (biology) , nymph , aphididae , aphis , aphis craccivora , mutualism (biology) , alate , population , virology , botany , symbiosis , homoptera , pest analysis , genetics , demography , sociology , bacteria
Vertically transmitted bacterial symbionts are common in arthropods. Aphids undergo an obligate symbiosis with Buchnera aphidicola , which provides essential amino acids to its host and contributes directly to nymph growth and reproduction. We previously found that newly adult Aphis glycines feeding on soybean infected with the beetle‐transmitted Bean pod mottle virus (BPMV) had significantly reduced fecundity. We hypothesized that the reduced fecundity was attributable to detrimental impacts of the virus on the aphid microbiome, namely Buchnera . To test this, mRNA sequencing and quantitative real‐time PCR were used to assay Buchnera transcript abundance and titre in A. glycines feeding on Soybean mosaic virus ‐infected, BPMV‐infected, and healthy soybean for up to 14 days. Our results indicated that Buchnera density was lower and ultimately suppressed in aphids feeding on virus‐infected soybean. While the decreased Buchnera titre may be associated with reduced aphid fecundity, additional mechanisms are probably involved. The present report begins to describe how interactions among insects, plants, and plant pathogens influence endosymbiont population dynamics.