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Chitin deacetylase family genes in the brown planthopper, N ilaparvata lugens ( H emiptera: D elphacidae)
Author(s) -
Xi Y.,
Pan P.L.,
Ye Y.X.,
Yu B.,
Zhang C.X.
Publication year - 2014
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/imb.12113
Subject(s) - biology , chitin , moulting , cuticle (hair) , insect , integument , midgut , brown planthopper , transcriptome , gene , trehalase , chitin synthase , ecdysone , botany , microbiology and biotechnology , gene expression , biochemistry , genetics , enzyme , larva , chitosan
Abstract Chitin deacetylases ( CDAs ) are enzymes required for one of the pathways of chitin degradation, in which chitosan is produced by the deacetylation of chitin. Bioinformatic investigations with genomic and transcriptomic databases identified four genes encoding CDAs in N ilaparvata lugens ( NlCDA s ). Phylogenetic analysis showed that insect CDA s were clustered into five major groups. Group I , III and IV CDA s are found in all insect species, whereas the pupa‐specific group II and gut‐specific group V CDA s are not found in the plant‐sap/blood‐sucking hemimetabolous species from H emiptera and A noplura. The developmental and tissue‐specific expression patterns of four NlCDA s revealed that NlCDA3 was a gut‐specific CDA , with high expression at all developmental stages; NlCDA1 , NlCDA2 and NlCDA4 were highly expressed in the integument and peaked periodically during every moulting, which suggests their roles in chitin turnover of the insect old cuticle. Lethal phenotypes of cuticle shedding failure and high mortality after the injection of double‐stranded RNAs ( dsRNAs) for NlCDA1 , NlCDA2 and NlCDA4 provide further evidence for their functions associated with moulting. No observable morphological and internal structural abnormality was obtained in insects treated with dsRNA for gut‐specific NlCDA3 .