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Two CYP4 genes of the C hinese white pine beetle, D endroctonus armandi ( C urculionidae: S colytinae), and their transcript levels under different development stages and treatments
Author(s) -
Dai L.,
Wang C.,
Zhang X.,
Yu J.,
Zhang R.,
Chen H.
Publication year - 2014
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/imb.12108
Subject(s) - bark beetle , curculionidae , dendroctonus , biology , host (biology) , insect , gene , pinene , bark (sound) , mountain pine beetle , phloem , botany , white (mutation) , cytochrome p450 , pheromone , enzyme , ecology , biochemistry
Bark beetles oxidize the defensive monoterpenes of their host trees both to detoxify them and to convert them into components of their pheromone system. This oxidation is catalysed by cytochrome P 450 ( CYP ) enzymes and occurs in different stages of the insect. We identified two new CYP4 genes in the C hinese white pine beetle ( D endroctonus armandi ), and carried out bioinformatic analysis one the full‐length nucleic acid sequences and deduced amino acid sequences. Differential expression of the CYP4 genes was observed between sexes, and within these significant differences amongst development stages, fed on phloem of P inus armandi and exposed to stimuli((±)‐ α‐pinene, ( R )‐(+)‐ α‐pinene, ( S )‐(−)‐α‐pinene, ( S )‐(−)‐β‐pinene and (+)‐3‐carene) at 8 and 24 h, and their interactions were found upon exposure to host monoterpenes. Increased expression of CYP4 genes suggested that they play a role in the detoxification of monoterpenes released by the host trees. The differential transcript accumulation patterns of these bark beetle CYP4 genes provides insight into the ecological interactions of D . armandi with its host pine.