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Response to cold acclimation in diapause pupae of H yles euphorbiae ( L epidoptera: S phingidae): candidate biomarker identification using proteomics
Author(s) -
Stuckas H.,
Mende M. B.,
Hundsdoerfer A. K.
Publication year - 2014
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/imb.12093
Subject(s) - biology , proteomics , acclimatization , diapause , botany , food science , biochemistry , larva , gene
The distribution range of H yles euphorbiae covers distinct climates across the P alaearctic. Previous investigations showed a correlation between mitochondrial DNA identity of populations and climatic conditions related to winter; however, the lack of biomarkers hampers investigations to test whether geographically distinct populations do show specific molecular responses to low temperatures or whether they possess specific genetic identity at loci functionally related to cold response. The present study was designed to identify candidate protein biomarkers and biological processes that are associated with cold acclimation of overwintering H . euphorbiae diapause pupae. Specimens taken from a single central E uropean population were gradually cooled from 20 °C to −2 °C over 36 days and 12 differentially abundant proteins were identified. In addition, D eep S uper SAGE sequencing technology was applied to study differentially regulated genes. There was incongruence between differentially abundant proteins and differentially expressed genes, but functional characteristics of regulated proteins and analyses of gene ontology term enrichment among differentially regulated genes pointed to activation of the same biological processes, e.g. oxidative stress response. As proteins represent biologically active molecules, candidate biomarkers derived from proteomics are considered well suited to explore intraspecific patterns of local adaptation to different climates.