Premium
ER type I signal peptidase subunit ( LmSPC 1) is essential for the survival of L ocusta migratoria manilensis and affects moulting, feeding, reproduction and embryonic development
Author(s) -
Zhang W.,
Xia Y.
Publication year - 2014
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/imb.12080
Subject(s) - biology , endoplasmic reticulum , kdel , signal peptide , rna interference , moulting , er retention , microbiology and biotechnology , midgut , secretion , secretory protein , unfolded protein response , biochemistry , rna , gene , peptide sequence , botany , mutant , golgi apparatus , larva
The endoplasmic reticulum type I signal peptidase complex ( ER SPC ) is a conserved enzyme that cleaves the signal peptides of secretory or membrane preproteins. The deletion of this enzyme leads to the accumulation of uncleaved proteins in biomembranes and cell death. However, the physiological functions of ER SPC in insects are not fully understood. Here, a catalytic subunit gene of ER SPC , LmSPC 1, was cloned from L ocusta migratoria manilensis and its physiological functions were analysed by RNA interference ( RNAi ). The LmSPC 1 open reading frame encoded a protein of 178 amino acids with all five conserved regions of signal peptidases. RNAi ‐mediated knockdown of LmSPC 1 resulted in high mortality. Sixty‐nine per cent of dead nymphs died of abnormal moulting, corresponding to decreased activity of moulting fluid protease. Moreover, insects in the RNAi group experienced a decline in food intake, and a decrease in the secretion of total protein and digestive enzymes from midgut tissues to the midgut lumen. Furthermore, the females produced fewer eggs and eggs with disrupted embryogenesis. These results indicate that LmSPC 1 is required for the secretion of secretory proteins, affects physiological functions, including moulting, feeding, reproduction and embryonic development, and is essential for survival. Therefore, LmSPC 1 may be a potential target for locust control.