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Differential transcription profiles in A edes aegypti detoxification genes after temephos selection
Author(s) -
SaavedraRodriguez K.,
Strode C.,
Flores A. E.,
GarciaLuna S.,
ReyesSolis G.,
Ranson H.,
Hemingway J.,
Black W. C.
Publication year - 2014
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/imb.12073
Subject(s) - biology , aedes aegypti , gene , esterase , glutathione s transferase , genetics , glutathione , enzyme , biochemistry , botany , larva
The mosquito A edes aegypti is the main vector of D engue and Y ellow F ever flaviviruses. The organophosphate insecticide temephos is a larvicide that is used globally to control A e. aegypti populations; many of which have in turn evolved resistance. Target site alteration in the acetylcholine esterase of this species has not being identified. Instead, we tracked changes in transcription of metabolic detoxification genes using the A e. aegypti ‘ D etox C hip ’ microarray during five generations of temephos selection. We selected for temephos resistance in three replicates in each of six collections, five from M exico, and one from P eru. The response to selection was tracked in terms of lethal concentrations. Uniform upregulation was seen in the epsilon class glutathione‐ S ‐transferase ( eGST ) genes in strains from M exico prior to laboratory selection, while eGSTs in the I quitos P eru strain became upregulated after five generations of temephos selection. While expression of many carboxyl/cholinesterase esterase ( CCE ) genes increased with selection, no single esterase was consistently upregulated and this same pattern was noted in the cytochrome P 450 monooxygenase ( CYP ) genes and in other genes involved in reduction or oxidation of xenobiotics. Bioassays using glutathione‐ S ‐transferase ( GST) , CCE and CYP inhibitors suggest that various CCEs instead of GSTs are the main metabolic mechanism conferring resistance to temephos. We show that temephos‐selected strains show no cross resistance to permethrin and that genes associated with temephos selection are largely independent of those selected with permethrin in a previous study.