Premium
Cloning and expression profiling of odorant‐binding proteins in the tarnished plant bug, L ygus lineolaris
Author(s) -
Hull J. J.,
Perera O. P.,
Snodgrass G. L.
Publication year - 2014
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/imb.12064
Subject(s) - lygus , biology , tarnished plant bug , genetics , conserved sequence , sequence alignment , odorant binding protein , gene , computational biology , peptide sequence , botany , hemiptera , miridae
Abstract In insects, the perception and discrimination of odorants requires the involvement of odorant‐binding proteins ( OBPs ). To gain a better molecular understanding of olfaction in the agronomic pest L ygus lineolaris (the tarnished plant bug), we used a transcriptomics‐based approach to identify potential OBPs . In total, 33 putative OBP transcripts, including the previously reported L ygus antennal protein ( LAP ), were identified based on the characteristic OBP Cys signature and/or sequence similarity with annotated orthologous sequences. The L . lineolaris OBP ( LylinOBP ) repertoire consists of 20 ‘classic’ OBPs , defined by the spacing of six conserved Cys residues, and 12 ‘ P lus‐ C ’ OBPs , defined by the spacing of eight conserved Cys and one conserved P ro residue. Alternative splicing of OBP genes appears to contribute significantly to the multiplicity of LylinOBP sequences. Microarray‐based analysis of chemosensory tissues (antennae, legs and proboscis) revealed enrichment of 21 LylinOBP transcripts in antennae, 12 in legs, and 15 in proboscis, suggesting potential roles in olfaction and gustation respectively. PCR ‐based determination of transcript abundance for a subset of the LylinOBP genes across multiple adult tissues yielded results consistent with the hybridization data.