z-logo
Premium
Genome‐wide screening for components of small interfering RNA ( siRNA) and micro‐RNA ( miRNA) pathways in the brown planthopper, N ilaparvata lugens ( H emiptera: D elphacidae)
Author(s) -
Xu HJ.,
Chen T.,
Ma XF.,
Xue J.,
Pan PL.,
Zhang XC.,
Cheng JA.,
Zhang CX.
Publication year - 2013
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/imb.12051
Subject(s) - brown planthopper , rna interference , biology , rna silencing , small interfering rna , gene , dicer , delphacidae , microrna , genetics , rna , small rna , transcriptome , gene silencing , trans acting sirna , argonaute , microbiology and biotechnology , gene expression , pest analysis , botany , homoptera
The brown planthopper ( BPH ), N ilaparvata lugens , is a major rice pest in Asia, and accumulated evidence indicates that this species is susceptible to RNA interference ( RNAi ); however, the mechanism underlying RNAi and parental RNAi has not yet been determined. We comprehensively investigated the repertoire of core genes involved in small interfering RNA ( siRNA) and micro‐RNA ( miRNA) pathways in the BPH by comparing its newly assembled transcriptome and genome with those of D rosophila melanogaster , T ribolium castaneum and C aenorhabditis elegans . Our analysis showed that the BPH possesses one drosha and two D icer ( dcr ) genes, three dsRNA ‐binding motif protein genes, two Argonaute ( ago ) genes, two E ri‐1 ‐like genes ( eri‐1 ), and a S id‐1 ‐like gene ( sid‐1 ). Additionally, we report for first time that parental RNAi might occur in this species, and siRNA pathway and S id‐1 were required for high efficiency of systemic RNAi triggered by exogenous dsRNA . Furthermore, our results also demonstrated that the miRNA pathway was involved in BPH metamorphosis as depletion of the ago1 or dcr1 gene severely impaired ecdysis. The BPH might be a good model system to study the molecular mechanism of systemic RNAi in hemimetabolous insects, and RNAi has potential to be developed to control this pest in agricultural settings.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here