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Genome‐wide screening for components of small interfering RNA ( siRNA) and micro‐RNA ( miRNA) pathways in the brown planthopper, N ilaparvata lugens ( H emiptera: D elphacidae)
Author(s) -
Xu HJ.,
Chen T.,
Ma XF.,
Xue J.,
Pan PL.,
Zhang XC.,
Cheng JA.,
Zhang CX.
Publication year - 2013
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/imb.12051
Subject(s) - brown planthopper , rna interference , biology , rna silencing , small interfering rna , gene , dicer , delphacidae , microrna , genetics , rna , small rna , transcriptome , gene silencing , trans acting sirna , argonaute , microbiology and biotechnology , gene expression , pest analysis , botany , homoptera
The brown planthopper ( BPH ), N ilaparvata lugens , is a major rice pest in Asia, and accumulated evidence indicates that this species is susceptible to RNA interference ( RNAi ); however, the mechanism underlying RNAi and parental RNAi has not yet been determined. We comprehensively investigated the repertoire of core genes involved in small interfering RNA ( siRNA) and micro‐RNA ( miRNA) pathways in the BPH by comparing its newly assembled transcriptome and genome with those of D rosophila melanogaster , T ribolium castaneum and C aenorhabditis elegans . Our analysis showed that the BPH possesses one drosha and two D icer ( dcr ) genes, three dsRNA ‐binding motif protein genes, two Argonaute ( ago ) genes, two E ri‐1 ‐like genes ( eri‐1 ), and a S id‐1 ‐like gene ( sid‐1 ). Additionally, we report for first time that parental RNAi might occur in this species, and siRNA pathway and S id‐1 were required for high efficiency of systemic RNAi triggered by exogenous dsRNA . Furthermore, our results also demonstrated that the miRNA pathway was involved in BPH metamorphosis as depletion of the ago1 or dcr1 gene severely impaired ecdysis. The BPH might be a good model system to study the molecular mechanism of systemic RNAi in hemimetabolous insects, and RNAi has potential to be developed to control this pest in agricultural settings.