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I n silico cloning and annotation of genes involved in the digestion, detoxification and RNA interference mechanism in the midgut of B actrocera dorsalis [ H endel ( D iptera: T ephritidae)]
Author(s) -
Shen G.M.,
Dou W.,
Huang Y.,
Jiang X.Z.,
Smagghe G.,
Wang J.J.
Publication year - 2013
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/imb.12026
Subject(s) - biology , midgut , rna interference , transcriptome , gene , genetics , drosophila melanogaster , in silico , bactrocera dorsalis , tephritidae , pest analysis , rna , botany , gene expression , larva
As the second largest organ in insects, the insect midgut is the major tissue involved in the digestion of food and detoxification of xenobiotics, such as insecticides, and the first barrier and target for oral RNA interference ( RNAi) . In this study, we performed a midgut‐specific transcriptome analysis in the oriental fruit fly, B actrocera dorsalis , an economically important worldwide pest, with many populations showing high levels of insecticide resistance. Using high‐throughput sequencing, 52 838 060 short reads were generated and assembled to 25 236 unigenes with a mean length of 758 bp. Interestingly, 34 unique sequences encoding digestion enzymes were newly described and these included aminopeptidase and trypsin, genes associated with B acillus thuringiensis resistance and fitness cost. Second, 41 transcripts were annotated to particular detoxification genes such as glutathione S ‐ transferases, carboxylesterases and cytochrome P 450s, and the subsequent phylogenetic analysis indicated homology with tissue‐specific and insecticide resistance‐related genes of D rosophila melanogaster . Third, we identified the genes involved in the mechanism of RNAi and the uptake of double‐stranded RNA . The sequences encoding D icer‐2, R2D2 , AGO2 , and E ater were confirmed, but SID and SR‐CI were absent in the midgut transcriptome. In conclusion, the results provide basic molecular information to better understand the mechanisms of food digestion, insecticide resistance and oral RNAi in this important pest insect in agriculture. Specific genes in these systems can be used in the future as potential targets for pest control, for instance, with RNAi technology.