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Analysis of gene transcription and relative abundance of the cys‐motif gene family from C ampoletis sonorensis ichnovirus ( CsIV ) and further characterization of the most abundant cys‐motif protein, WH v1.6
Author(s) -
Gill T. A.,
Webb B. A.
Publication year - 2013
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/imb.12022
Subject(s) - gene , biology , gene expression , microbiology and biotechnology , gene family , genetics
The cys‐motif gene family associated with C ampoletis sonorensis ichnovirus contains 10 members, WH v1.6, WH v1.0, VH v1.1, VH v1.4, AH v1.0, A'H v0.8, FH v1.4, LH v2.8, UH v0.8, and UH v0.8a . The results of this study indicated that, within the encapsidated virion, WH v1.6 is the most abundant cys‐motif gene, while the combined AHv genes are the least abundant. During parasitization of H eliothis virescens by C ampoletis sonorenis , WH v1.6 transcripts were the mostly highly expressed, while the combined UHv genes had the lowest expression. Further proteomic analysis of WH v1.6 showed that it accumulates at high levels in parasitized plasma by 6 h, and is detectable in the haemocytes, fat body, malpighian tubules, nerve cord and epidermis by 2 days after parasitization. Localization experiments led us to conclude that WH v1.6 interacts with the cell membrane along with other organelles within a virus‐infected cell and prevents immunocytes from spreading or adhering to a foreign surface. Similarly to VH v1.4 and VH v1.1, WH v1.6 is able to inhibit the translation of haemocyte and Malpighian tubule RNAs . Our results showed that the expression of cys‐motif genes during parasitization is related to the gene copy number of each gene within the encapsidated virion and may also be dependent upon cis‐regulatory element activity in different target tissues. In addition, WH v1.6 plays a major role in inhibiting the cellular encapsulation response by H . virescens .