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Long‐term administration of alpha‐1 blocker can reverse the micturition pattern in a bladder outlet obstruction murine model
Author(s) -
Kitta Takeya,
Chiba Hiroki,
KannoKakibuchi Yukiko,
Hattori Tsuyoshi,
Higuchi Madoka,
Ouchi Mifuka,
Togo Mio,
AbeTakahashi Yui,
Michishita Mai,
Kitano Tatsuya,
Shinohara Nobuo
Publication year - 2020
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/iju.14377
Subject(s) - bladder outlet obstruction , urination , medicine , urology , urethra , urinary bladder neck obstruction , urinary bladder , urinary system , prostate , cancer
Objective To investigate the effect of chronic administration of an alpha‐1 blocker on micturition patterns in long‐term partial bladder outlet obstruction. Methods Mice were divided into three groups: a normal group, in which animals were fed a standard diet; a partial bladder outlet obstruction group, in which the proximal urethra was tied and animals were fed a standard diet; and a partial bladder outlet obstruction + naftopidil group, in which the proximal urethra was tied and animals were fed a standard diet containing naftopidil. Micturition behavior was evaluated in all groups for 6 months after partial bladder outlet obstruction surgery. The parameters evaluated included voided volume, time per void, urination frequency and total urine volume. Quantitative assessment of gene expression was also carried out. Results Total urine volume, as well as total and average voided volume during night, was significantly decreased in partial bladder outlet obstruction + naftopidil mice compared with partial bladder outlet obstruction animals. The levels of transcripts encoding 5‐hydroxytryptamine 2A and tissue inhibitor of metalloproteinase 2 were significantly decreased in the partial bladder outlet obstruction + naftopidil group compared with the partial bladder outlet obstruction group. Conclusions Long‐term administration of an alpha‐1 blocker seems to reverse the disturbance of the micturition pattern caused by partial bladder outlet obstruction. Mechanistically, this effect might be mediated by changes in the expression of a serotonin receptor and/or in the activity of the fibrogenesis pathway.