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Spinal glycinergic and gamma‐aminobutyric acid‐ergic neurons inhibit the micturition reflex after electrical stimulation of the perineum in rats with pelvic venous congestion
Author(s) -
Sugaya Kimio,
Nishijima Saori,
Kadekawa Katsumi,
Noguchi Katsuhiko,
Ueda Tomoyuki,
Yamamoto Hideyuki
Publication year - 2019
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/iju.14116
Subject(s) - strychnine , medicine , glycine receptor , stimulation , urinary bladder , bicuculline , anesthesia , spinal cord , perineum , cystometry , endocrinology , antagonist , receptor , pharmacology , anatomy , glycine , chemistry , biochemistry , amino acid , psychiatry
Objectives To examine whether electrical stimulation of the perineum inhibited urinary frequency in rats with pelvic venous congestion, and whether electrical stimulation influences spinal glycinergic/gamma‐aminobutyric acid‐ergic neurons. Methods Bilateral common iliac veins and bilateral uterine veins were ligated to create pelvic venous congestion rats. At 4 weeks after ligation, cystometry was carried out before and after electrical stimulation with/without intrathecal injection of strychnine (a glycine receptor antagonist) and/or bicuculline (a gamma‐aminobutyric acid type A receptor antagonist). In addition, measurement of amino acid levels in the lumbosacral cord was carried out with/without electrical stimulation, and cystometry was carried out after oral administration of glycine. Results Continuous cystometry showed that the interval between bladder contractions was shorter in pelvic venous congestion rats than in sham rats. Electrical stimulation did not change cystometric parameters in sham rats, but the interval between bladder contractions was increased by electrical stimulation in pelvic venous congestion rats. Electrical stimulation increased the levels of glutamic acid, glycine, gamma‐aminobutyric acid, and taurine in the lumbosacral cord of pelvic venous congestion rats. Intrathecal strychnine abolished the effects of electrical stimulation in pelvic venous congestion rats, and intrathecal administration of both strychnine and bicuculline shortened the interval between bladder contractions more than before electrical stimulation. Oral administration of glycine (3%) to pelvic venous congestion rats increased bladder capacity. Conclusions Electrical stimulation of the perineum inhibits urinary frequency mainly through activation of spinal glycinergic neurons, and partly through activation of gamma‐aminobutyric acid‐ergic neurons in a rat model of pelvic venous congestion.