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Seminal microbiome in men with and without prostatitis
Author(s) -
Mändar Reet,
Punab Margus,
Korrovits Paul,
Türk Silver,
Ausmees Kristo,
Lapp Eleri,
Preem JensKonrad,
Oopkaup Kristjan,
Salumets Andres,
Truu Jaak
Publication year - 2017
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/iju.13286
Subject(s) - firmicutes , bacteroidetes , semen , microbiome , prostatitis , proteobacteria , medicine , lactobacillus , actinobacteria , polymerase chain reaction , biology , microbiology and biotechnology , genetics , 16s ribosomal rna , andrology , bacteria , prostate , gene , cancer
Objectives To profile the seminal microbiome applying next generation sequencing. Methods Semen samples of 67 men were involved in the study (21 men with and 46 men without prostatitis). Seminal microbiomes were profiled applying the method that uses combinatorial sequence tags attached to polymerase chain reaction primers that amplify the ribosomal ribonucleic acid V6 region. Amplified polymerase chain reaction products were sequenced using an Illumina paired‐end protocol on HiSeq2000 platform. Results The most abundant phylum in semen was Firmicutes , comprising nearly half of the sequences found (median 41.7%, quartiles 28.5–47.2%) followed by Bacteroidetes , Proteobacteria and Actinobacteria . The counts of lactobacilli were higher in healthy men than prostatitis patients (27% [20.2–34.6%] vs 20.2% [4.9–25.0%]; P = 0.05), especially for Lactobacillus iners . Proteobacteria comprised higher proportions in prostatitis patients than healthy men. The species richness was higher in prostatitis patients than healthy men (inverted Simpson index 13.5 ± 5.8 vs 10.3 ± 4.0). Conclusions The semen of chronic prostatitis patients contains fewer health‐supporting lactobacilli, and has higher species diversity than that of healthy men. Firmicutes (especially lactobacilli), Bacteroidetes, Proteobacteria and Actinobacteria comprise the highest proportion of seminal microbiome.