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Intravesical hyaluronic acid treatment improves bacterial cystitis and reduces cystitis‐induced hypercontractility in rats
Author(s) -
Yıldız Nurdan,
Alpay Harika,
Tuğtepe Halil,
Özdemir Kumral Zarife Nigar,
Akakın Dilek,
İlki Arzu,
Şener Göksel,
Ç Yeğen Berrak
Publication year - 2015
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/iju.12752
Subject(s) - hyaluronic acid , medicine , superoxide dismutase , interstitial cystitis , catalase , myeloperoxidase , carbachol , pharmacology , saline , urinary bladder , urology , oxidative stress , inflammation , urinary system , stimulation , anatomy
Objective To investigate the effect of intravesical hyaluronic acid on Escherichia coli ‐induced cystitis and cystitis‐induced hypercontractility in rats. Methods Bacterial cystitis was induced in Wistar female rats by intravesical inoculation of E. coli . Isotonic saline was instilled in the control group ( n = 6). The rats were either non‐treated, treated with gentamycin (4 mg/kg, 5 days) or treated intravesically with hyaluronic acid (0.5 mL, 0.5%). On the eighth day, the bladder tissues were excised for histological examination, and the measurements of myeloperoxidase, superoxide dismutase and catalase activities. Contraction/relaxation responses to carbachol, isoprotrenol and papaverine were studied. Results Tissue myeloperoxidase activity was increased, but superoxide dismutase and catalase activities were decreased in bacterial cystitis, while hyaluronic acid treatment reversed these changes. In the hyaluronic acid‐treated group, healing of the uroepithelium was observed, while decreased inflammatory cell infiltration was obvious in gentamycin‐treated group. E. coli ‐induced cystitis in all rats resulted in increased contraction responses to carbachol compared with controls ( P < 0.01). Treatment with hyaluronic acid, but not gentamycin, significantly ( P < 0.05) depressed hypercontractility at maximum carbachol concentrations. In all rats with cystitis, papaverine‐induced relaxation was increased, whereas isoproterenol‐induced relaxation curves were not different between the studied groups. Conclusion Gentamycin treatment, despite its ameliorative effect on inflammation, had no impact on the contractile dysfunction of the injured bladder. Intravesical hyaluronic acid, in addition to its supportive role in the healing of the epithelium, seems to lower the increased threshold for contraction and to reduce oxidative stress. These findings support a potential role for hyaluronic acid in the treatment of bacterial cystitis.