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Gut microbiota in adolescent girls with polycystic ovary syndrome: Effects of randomized treatments
Author(s) -
GarciaBeltran Cristina,
Malpique Rita,
Carbonetto Belen,
GonzálezTorres Pedro,
Henares Desirée,
Brotons Pedro,
MuñozAlmagro Carmen,
LópezBermejo Abel,
Zegher Francis,
Ibáñez Lourdes
Publication year - 2021
Publication title -
pediatric obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.226
H-Index - 69
eISSN - 2047-6310
pISSN - 2047-6302
DOI - 10.1111/ijpo.12734
Subject(s) - polycystic ovary , prevotella , medicine , gut flora , body mass index , endocrinology , physiology , hyperandrogenism , obesity , biology , insulin resistance , genetics , immunology , bacteria
Summary Background Girls with obesity and polycystic ovary syndrome (PCOS) and women with PCOS have altered gut microbiota. Objective To study the gut microbiota composition of girls with PCOS without obesity (age, 15.8 years; body mass index [BMI] 25 kg/m 2 ) and the effects of randomized treatments with an oral contraceptive (OC, N = 15) or with spironolactone‐pioglitazone‐metformin (SPIOMET, N = 15) for 1 year. Thirty‐one age‐matched girls served as controls. Methods 16S ribosomal subunit gene amplicon sequencing was performed in stool samples from all subjects; samples from 23 out of 30 girls with PCOS (OC, N = 12; SPIOMET, N = 11) were available for analysis post‐treatment. Clinical and endocrine‐metabolic variables were measured before and after intervention. Results Girls with PCOS had decreased diversity alpha, altered microbiota pattern and taxonomic profile with more abundance of Family XI ( P = .002), and less abundance of family Prevotellaceae ( P = .0006) the genus Prevotella ( P = .0001) and Senegalimassilia ( P < .0001), as compared to controls. Family XI abundance related positively to hepato‐visceral fat (R = 0.453; P = .0003). SPIOMET treatment, but not OC, normalized the abundance of Family XI . Prevotellaceae , Prevotella and Senegalimassilia abundance remained unchanged after either treatment. Conclusion SPIOMET's spectrum of normalizing effects in girls with PCOS is herewith broadened as to include Family XI abundance in gut microbiota.