Premium
Association of AMY1A / AMY2A copy numbers and AMY1 / AMY2 serum enzymatic activity with obesity in Mexican children
Author(s) -
VázquezMoreno Miguel,
MejíaBenítez Aurora,
Sharma Tanmay,
PeraltaRomero Jesús,
LociaMorales Daniel,
KlünderKlünder Miguel,
Cruz Miguel,
Meyre David
Publication year - 2020
Publication title -
pediatric obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.226
H-Index - 69
eISSN - 2047-6310
pISSN - 2047-6302
DOI - 10.1111/ijpo.12641
Subject(s) - obesity , medicine , amylase , enzyme assay , anti obesity , starch , alpha amylase , enzyme , food science , endocrinology , biology , biochemistry
SUMMARY Background Mexican children are characterized by a high‐starch intake diet and high prevalence of obesity. Objectives To investigate the association of AMY1A / AMY2A copy numbers (CNs) and AMY1/AMY2 serum enzymatic activity with childhood obesity in up to 427 and 337 Mexican cases and controls. Methods Anthropometric and dietary starch intake data were collected. CN of AMY1A / AMY2A and AMY1/AMY2 serum enzymatic activity were determined using droplet digital PCR (ddPCR) and enzymatic colorimetry, respectively. An individual participant level data meta‐analysis of association between AMY1A CNVs and obesity was also performed. Results A positive association between AMY1A / AMY2A CNs and their corresponding AMY1/AMY2 serum enzyme activity was observed in children with normal weight and obesity. The serum enzyme activity of AMY1 and AMY2 was negatively associated with childhood obesity risk, and the association was restricted to kids eating medium/high amount of starch ( P interaction = .004). While no association between AMY1A and AMY2A CNs and childhood obesity was observed in our sample, we confirmed a significant association between AMY1A CN and obesity in a meta‐analysis of 3100 Mexican children. Conclusions Our data suggest that genetically determined salivary and pancreatic amylase activity can increase/decrease the risk of obesity in Mexican children, this effect being blunted by a low‐starch diet.