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The relative expression levels of CD148 and CD180 on clonal B cells and CD148/CD180 median fluorescence intensity ratios are useful in the characterization of mature B cell lymphoid neoplasms infiltrating blood and bone marrow – Results from a single centre pilot study
Author(s) -
Gautam Arambam,
Sreedharanunni Sreejesh,
Sachdeva Man Updesh Singh,
Rana Sonia,
Kashyap Dharambir,
Bose Parveen,
Bal Amanjeet,
Prakash Gaurav,
Malhotra Pankaj,
Das Reena,
Varma Neelam
Publication year - 2021
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.13467
Subject(s) - flow cytometry , medicine , lymphoma , mantle cell lymphoma , microbiology and biotechnology , pathology , gastroenterology , biology , immunology
The categorization of mature B cell neoplasms (MBN) infiltrating blood and bone marrow are met with difficulties. The inclusion of CD148 and CD180 in the routine flow cytometry/FCM panels has been suggested to refine the diagnosis. We studied the discriminatory ability of CD148 and CD180 median fluorescence intensity(MFI), CD148/CD180 ratio and their expression relative to T cells (CD148 ab/T , CD180 ab/T ), neutrophils (CD148 ab/gr , CD180 ab/gr ) and normal B cells (CD148 ab/n , CD180 ab/n ) in the differentiation of mature B cell neoplasms (MBN) especially non‐chronic lymphocytic leukaemia (CLL). Methodology The flow cytometric (FCM) expression of CD148 and CD180 was studied prospectively in 102 patients (non‐CLL; n = 72); diagnosed by a comprehensive panel of immunophenotypic and cytogenetic studies. The MFI and ratios were statistically compared across MBNs by Mann‐Whitney U test. Cut‐off values, sensitivity and specificity were calculated for significant parameters by receiver operator characteristic curve. Results CD180MFI > 4.35 showed 100% sensitivity and 90.9% specificity for a diagnosis of marginal zone lymphoma (MZL) while, CD148/180 > 5.15 was 100% specific and 81.8% sensitive for lymphoplasmacytic lymphoma. CD148 ab/T (>4.3; 100% specificity, 83.4% sensitivity) and CD148 ab/gr (>1.1; 100% sensitivity, 90% sensitivity) were useful for differentiating blastoid‐mantle cell lymphoma/MCL from diffuse large B cell lymphoma; while CD148MFI (≥20.25), CD148 ab/T (>3.35) and CD148 ab/gr (>0.95) showed >90% specificity and sensitivity for distinguishing MCL from CLL. Pairwise analysis also showed a good discriminant function of various parameters for distinguishing SMZL from other MBNs like FL, MCL as well as CLL. Conclusions The current study shows an excellent utility of CD148MFI, CD180MFI, their ratio and relative expression levels in the subcategorization of immunophenotypically related MBNs.