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A study to compare Hematopoietic Progenitor Cell count determined on a next‐generation automated cell counter with flow cytometric CD34 count in peripheral blood and the harvested peripheral blood stem cell graft from autologous and allogenic donors
Author(s) -
Mishra Shruti,
Kulkarni Uday,
Mathews Nitty,
V Ramya,
Ch. Nair Sukesh,
George Biju,
Mammen Joy J.
Publication year - 2021
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.13341
Subject(s) - cd34 , apheresis , peripheral blood mononuclear cell , progenitor cell , flow cytometry , medicine , haematopoiesis , blood cell , bone marrow , leukapheresis , peripheral blood , white blood cell , stem cell , immunology , andrology , biology , platelet , in vitro , biochemistry , genetics
Abstract Introduction A successful bone marrow transplant requires a minimum of 2‐4 × 10 6 cells/kg patient body weight of CD 34+ cells to be transfused, where peripheral blood CD34+ cell count being and ideal predictor. We compared the correlation and predictive capacity of both hematopoietic progenitor cell count (HPC) determined on the Sysmex XN‐9000 and flow cytometric CD34 in autologous and allogenic donors. Methods Autologous and allogenic donors were taken as per criteria. TLC (Total Leukocyte Count), MNC (Mononuclear cell count), HPC, and CD34 assay were done in both the peripheral blood prior to apheresis, and the harvest product postapheresis. Sysmex XN‐9000 was used for TLC, MNC, and HPC tests, and a modified ISH‐AGE protocol was used to enumerate CD34 by flow cytometry. Statistical analysis was done using SPSS 16.0. Results Sixty‐seven allogenic and 35 autologous donors were enrolled. 45% were females, and 55% were males. Correlation between HPC and CD34 was found to be 0.887 with P value < .01 in peripheral blood and 0.847 with P value < .01 in the harvested product. On the other hand, TLC had a correlation of 0.424 and 0.520 in peripheral blood and harvested, respectively. MNC had a weak association. The cutoff value for a target dose of 2 × 10 6 CD34 cells/kg was 37 × 10 6 /L for pre‐HPC. For a target of 4 × 10 6 CD34 cells/kg, the cutoff value calculated to be 54 × 10 6 /L (Sensitivity: 85%, Specificity: 89%) for peripheral blood HPC. Conclusion We conclude that HPC is comparable to CD34 in predicting harvest product's adequacy.