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P‐glycoprotein and multidrug resistance‐associated protein‐1 expression in acute myeloid leukemia: Biological and prognosis implications
Author(s) -
da Silveira Júnior Lenilton Silva,
Soares Victor de Lima,
Jardim da Silva Alessandra Suelen,
Gil Erica Aires,
Pereira de Araújo Maria das Graças,
Merces Gonçalves Ciro Alexandre,
Paiva Aldair de Souza,
Moura de Oliveira Taissa Maria,
Oliveira Gustavo Henrique de Medeiros,
Kramer Cavacanti e Silva Dany Geraldo,
de Araújo Moura Lemos Telma Maria,
Moretti Rebecchi Ivanise Marina,
de Farias Sales Valeria Soraya,
Luchessi André Ducati,
Cavalcanti Júnior Geraldo Barroso
Publication year - 2020
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.13241
Subject(s) - myeloid leukemia , flow cytometry , multiple drug resistance , p glycoprotein , cd34 , leukemia , medicine , disease , bone marrow , myeloid , immunology , haematopoiesis , oncology , drug resistance , cancer research , biology , stem cell , genetics , microbiology and biotechnology
Abstract Background Despite the advances in the cure rate for acute myeloid leukemia (AML), a considerable number of patients die from the disease due to the occurrence of multidrug resistance (MDR). Overexpression of the transporter proteins, such as P‐glycoprotein (Pgp) and multidrug resistance‐associated protein (MRP), confers resistance to the treatment of these leukemias. Methods To analyze the expression of the Pgp and MRP1 in patients with AML and determine their correlation between expression and demographic, clinical, and laboratorial variables, bone marrow and peripheral blood samples from 346 patients with a diagnosis of AML were assessed for the expression of Pgp and MRP1 by flow cytometry. Results The expression of Pgp and MRP1 was found in 111 (32.1%) and 133 (38.4%) patients, respectively, with greater prevalence in older patients and lower in children, while also observing a high incidence in patients with refractory, recurrence, and secondary disease in comparison with the cases of de novo AML. Regarding the laboratory findings, we observed an association between the expression of Pgp and MRP1 and CD34, CD7, and also M7, M5a, and M2‐AML of French‐American‐British classification. Conclusions The results showed that the detection of MDR phenotype by flow cytometry can be a molecular marker for prognosis of patients with AML.