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FLAER‐negative CD15+ neutrophils can be used for the simplified screening of suspected PNH cases
Author(s) -
Semchenkova Alexandra,
Kashpor Svetlana,
Verzhbitskaya Tatiana,
Borisov Vyacheslav,
Illarionova Olga,
Fechina Larisa,
Maschan Alexey,
Novichkova Galina,
Popov Alexander
Publication year - 2020
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.13239
Subject(s) - cd15 , negativity effect , false positive paradox , flow cytometry , medicine , cd64 , immunology , psychology , computer science , biology , social psychology , artificial intelligence , genetics , stem cell , cd34
Background The flow cytometry analysis of GPI‐linked proteins on red blood cells and leukocytes is crucial for paroxysmal nocturnal hemoglobinuria (PNH) diagnostics. However, the commonly used multicolor panels cannot be implemented in low‐resourced hematology laboratories. In order to develop a simple prediagnostic test for PNH screening, we analyzed the diagnostic accuracy of the two‐color (FLAER/CD15) detection of GPI‐deficient neutrophils. Methods We reanalyzed multicolor data set of 1594 peripheral blood samples of patients screened for PNH applying only two markers (FLAER/CD15). The quantitative positivity/negativity was reported. Then, these results were compared in a blinded manner with previously obtained multicolor data from the same samples. Results Among the 1594 samples included in the study, 507 samples were PNH‐positive by the multicolor assay. The two‐color method revealed 510 PNH‐positive samples. The detailed examination of this discrepancy revealed 12 false‐positives and 9 false‐negatives. Therefore, FLAER/CD15 screening method displayed 98.90% of the diagnostic specificity and 98.22% of the sensitivity. Conclusion This simple two‐color evaluation of FLAER‐negative neutrophils is a highly effective screening test for PNH. Although this approach is not intended to replace the multicolor diagnostic procedure, it could minimize the number of patients requiring a conventional multicolor flow cytometric assay.