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Flow cytometry identification of nonhemopoietic neoplasms during routine immunophenotyping
Author(s) -
Stacchini Alessandra,
Aliberti Sabrina,
Demurtas Anna,
Maletta Francesca,
Molinaro Luca,
Godio Laura,
Papotti Mauro
Publication year - 2019
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12946
Subject(s) - immunophenotyping , flow cytometry , identification (biology) , medicine , pathology , biology , immunology , botany
Nonhemopoietic neoplasms ( NHN s) may be encountered during routine flow cytometry ( FC ) immunophenotyping. The clue of their presence mainly relies on detection of CD45‐negative ( CD 45−) cells with altered scatter parameters. Methods In this study, we evaluated a monoclonal antibody combination conceived to characterize the CD 45− population by FC , suspected of belonging to NHN s, when present. The panel included CD 45 for leucocytes identification, CD 326 (clones Ber EP 4 and HEA ‐125) to mark epithelial cells, CD 33 to identify myeloid cells, CD 138 to trace plasma cells and CD 56 useful in the identification of neuroendocrine tumours. 7 AAD vital dye was used to gate out dead cells. Results were correlated with cytomorphology and confirmed by histological data, if available. Results Among 9422 specimens submitted for routine FC investigation, 47 samples that included fine‐needle aspirates, bone marrow aspirates, tissue biopsies and body fluids had a detectable CD 45− population and a sufficient cell amount to be further investigated. FC revealed the presence of CD 326‐positive epithelial cells in 38 specimens; altered scatter parameters and variable reactivity to the other antigens tested allowed to suspect NHN s in the remaining nine samples. The presence of NHN s was confirmed in all cases by morphology. Conclusions The current results show that when CD 45− cells with altered scatter parameters were detected, cytometrists involved in leukaemia/lymphoma diagnosis may require further FC investigations to rapidly identify NHN s in different specimens, thus reducing the time of the immunohistochemical diagnostic workup to reach a final diagnosis.