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Early diagnosis of infection occurs in burned patients and verification in vitro
Author(s) -
Zhang M.,
Yang L.,
Du G.,
Duan S.,
Liu D.,
Gao X.,
Li D.
Publication year - 2018
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12810
Subject(s) - cd64 , peripheral blood , medicine , in vitro , immunology , area under the curve , peripheral , gastroenterology , receiver operating characteristic , pathology , biology , flow cytometry , biochemistry
Objective To explore the trend of CD 64 + neutrophils being present in the peripheral blood of patients with burns and verification in vitro. Method We isolated and purified CD 64 + neutrophils from healthy people, stimulated cells with inactivated Staphylococcus aureus in vitro to make them proliferate and analyzed them with Modfit 2 analysis software. Patients with burns were divided into two groups, depending on whether there was a complicated infection or not, and 35 healthy participants were enrolled as blank group. WBC and CD 64 + neutrophil counts were detected and then analyzed the specificity and sensitivity according to the ROC curve. Results The proliferation index of the CD 64 + cells in the experimental wells was significantly higher than that in the control wells ( P < .05). The CD 64 + cell counts in the peripheral blood of patients in the infection group were significantly higher than patients without infection, but also higher than the healthy people ( P < .05). The sensitivity and specificity of CD 64 + cellular diagnosis for burn infection were 94.2% and 76.8%, respectively, according to the ROC curve, which were significantly better than those of the WBC s. Conclusion CD 64 + cells in the peripheral blood of patients with burns complicated by infection increased significantly, and thus, it can be regarded as an early diagnostic indicator of burns with a concurrent infection.