Premium
Platelet impedance adhesiometry: A novel technique for the measurement of platelet adhesion and spreading
Author(s) -
Polgár L.,
Soós P.,
Lajkó E.,
Láng O.,
Merkely B.,
Kőhidai L.
Publication year - 2018
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12799
Subject(s) - platelet , epinephrine , adhesion , thrombus , medicine , platelet adhesion , platelet activation , cell adhesion , immunology , pharmacology , chemistry , organic chemistry
Thrombogenesis plays an important role in today's morbidity and mortality. Antithrombotics are among the most frequently prescribed drugs. Thorough knowledge of platelet function is needed for optimal clinical care. Platelet adhesion is a separate subprocess of platelet thrombus formation; still, no well‐standardized technique for the isolated measurement of platelet adhesion exists. Impedimetry is one of the most reliable, state‐of‐art techniques to analyze cell adhesion, proliferation, viability, and cytotoxicity. We propose impedimetry as a feasible novel method for the isolated measurement of 2 significant platelet functions: adhesion and spreading. Methods Laboratory reference platelet agonists (epinephrine, ADP , and collagen) were applied to characterize platelet functions by impedimetry using the xCELL igence SP system. Platelet samples were obtained from 20 healthy patients under no drug therapy. Standard laboratory parameters and clinical patient history were also analyzed. Results Epinephrine and ADP increased platelet adhesion in a concentration‐dependent manner, while collagen tended to have a negative effect. Serum sodium and calcium levels and age had a negative correlation with platelet adhesion induced by epinephrine and ADP , while increased immunoreactivity connected with allergic diseases was associated with increased platelet adhesion induced by epinephrine and ADP . ADP increased platelet spreading in a concentration‐dependent manner. Conclusion Impedimetry proved to be a useful and sensitive method for the qualitative and quantitated measurement of platelet adhesion, even differentiating between subgroups of a healthy population. This novel technique is offered as an important method in the further investigation of platelet function.