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Increased circulating microparticles in women with preeclampsia
Author(s) -
Zhang Y.,
Zhao C.,
Wei Y.,
Yang S.,
Cui C.,
Yang J.,
Zhang J.,
Qiao R.
Publication year - 2018
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12796
Subject(s) - preeclampsia , receiver operating characteristic , platelet , medicine , andrology , endothelial dysfunction , inflammation , endothelial stem cell , gastroenterology , endocrinology , pregnancy , chemistry , biology , biochemistry , genetics , in vitro
Preeclampsia ( PE ) is associated with hypercoagulability, endothelial dysfunction and inflammation, which generate microparticles ( MP s). Therefore, MP s may be important for PE . Methods We established a verified MP measurement procedure to detect MP s in nonpregnant women (n = 25), healthy pregnant women (n = 29) and PE women (n = 73) and compared their MP levels. Results Microparticles prepared from platelets ( PMP s), endothelial cells ( EMP s) and leucocytes ( LMP s) were confirmed by transmission electron microscopy and were analysed by our established flow cytofluorimetric approach, which showed good specificity for determining the cell origin and level of MP s. The levels of total MP s ( tMP s) and PMP s in the healthy pregnant group were significantly higher than those in the nonpregnant group (158.78 vs 93.00 and 45.04 vs 17.41, P = .004 and P = .007, respectively) but were not significantly different from those of the PE group. However, EMP s and LMP s were significantly higher in the PE group than in the healthy pregnant group (14.62 vs 11.48 and 8.94 vs 5.03, P = .015 and P < .001, respectively). Furthermore, the area under the receiver operating characteristic curves ( AUC ) for EMP s, LMP s and the combined sum of EMP s and LMP s were 0.661, 0.746 and 0.718, respectively ( P < . 05); at their optimal cut‐off values, the sensitivities were 50.68%, 87.67% and 46.58%, respectively, and the specificities were 80.77%, 58.33% and 95.65%, respectively. Conclusion Determining the MP level, especially that of EMP s and LMP s, by a specificity‐verified method may reflect the endothelial dysfunction and inflammation involved in PE pathogenesis.