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A ten‐color tube with dried antibody reagents for the screening of hematological malignancies
Author(s) -
Correia R. P.,
Rajab A.,
Bento L. C.,
Alexandre A. M.,
Vaz A. C.,
Schimidell D.,
Pedro E. C.,
Perin F. S.,
Nozawa S. T.,
Barroso R. S.,
Bacal N. S.
Publication year - 2018
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/ijlh.12753
Subject(s) - pleural fluid , medicine , reagent , external quality assessment , chromatography , pathology , chemistry , pleural effusion
The workflow in clinical flow cytometry laboratories must constantly be reviewed to develop technical procedures that improve quality and productivity and reduce costs. Using the Beckman Coulter dry coating technology, we customized a ten‐color tube with dried antibody reagents, designated the Duraclone screening tube ( DST ), for screening hematological malignancies. Here, we compared the applicability, clinical and numerical equivalence, and cost and time required for the technical procedures between the liquid reagents and the DST . Methods The DST contains CD 4 + Kappa‐ FITC , CD 8 + Lambda‐ PE , CD 3 + CD 14‐ ECD , CD 33‐ PE ‐Cy5.5, CD 20 + CD 56‐ PE ‐Cy7, CD 34‐ APC , CD 19‐ APC ‐AlexaFluor700, CD 10‐ APC ‐AlexaFluor750, CD 5‐Pacific Blue, and CD 45‐Krome Orange. We evaluated 20 bone marrow samples, 13 peripheral blood samples, 6 lymph node biopsy samples, 5 fine‐needle aspirate samples, 5 cerebrospinal fluid samples, and 1 pleural fluid sample. Results The DST was useful for more than 60% of our samples. It was able to enumerate the majority of the populations in all types of samples with a statistically acceptable correlation with the liquid reagents. The use of the DST translated into significant time and cost savings of 15.8% and 12.3%, respectively, compared with the use of the liquid reagent. The cost was reduced by $14.36 per sample. Conclusions The DST is an efficient solution for screening hematological malignancies with improved quality, productivity, standardization, and sustainability. These improvements could benefit patients by providing faster diagnoses using a higher quality and lower cost reagent.